https://www.pistack.xyz/tags/variant-calling/ Recent content in Variant-Calling on Pi Stack Hugo en-us Wed, 10 Jun 2026 00:00:00 +0000 https://www.pistack.xyz/posts/2026-06-10-self-hosted-genomic-variant-calling-gatk-freebayes-bcftools/ Wed, 10 Jun 2026 00:00:00 +0000 https://www.pistack.xyz/posts/2026-06-10-self-hosted-genomic-variant-calling-gatk-freebayes-bcftools/ <h2 id="introduction">Introduction</h2> <p>Variant calling — the process of identifying genetic differences between a sequenced genome and a reference genome — is one of the most fundamental workflows in bioinformatics. Whether you&rsquo;re analyzing whole-genome sequencing (WGS), whole-exome sequencing (WES), or targeted gene panels, the choice of variant caller significantly impacts your downstream results.</p>