https://www.pistack.xyz/tags/rnaseq/ Recent content in Rnaseq on Pi Stack Hugo en-us Sat, 13 Jun 2026 00:00:00 +0000 https://www.pistack.xyz/posts/2026-06-13-self-hosted-rnaseq-alignment-quantification-star-kallisto-salmon-stringtie/ Sat, 13 Jun 2026 00:00:00 +0000 https://www.pistack.xyz/posts/2026-06-13-self-hosted-rnaseq-alignment-quantification-star-kallisto-salmon-stringtie/ <h2 id="introduction">Introduction</h2> <p>RNA sequencing (RNA-seq) has revolutionized our understanding of gene expression, transcript structure, and RNA biology. But the raw output from a sequencer — millions of short nucleotide reads — is useless without computational processing. Two critical steps transform raw reads into biologically meaningful data: alignment (mapping reads to a reference genome) and quantification (estimating transcript abundance).</p>