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    <title>Genome-Assembly on Pi Stack</title>
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      <title>Self-Hosted Genome Assembly Pipelines: SPAdes vs Canu vs Flye vs Hifiasm</title>
      <link>https://www.pistack.xyz/posts/2026-06-09-self-hosted-genome-assembly-spades-canu-flye-hifiasm-guide/</link>
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      <description>&lt;h2 id=&#34;introduction&#34;&gt;Introduction&lt;/h2&gt;&#xA;&lt;p&gt;Genome assembly — reconstructing complete DNA sequences from short or long sequencing reads — is the foundation of modern genomics research. Whether you&amp;rsquo;re studying antibiotic resistance in bacteria, assembling a new plant reference genome, or analyzing cancer mutations, the first computational step is always assembly. Running these tools on your own infrastructure gives you control over data privacy, compute resources, and pipeline customization.&lt;/p&gt;</description>
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