https://www.pistack.xyz/tags/cryo-em/ Recent content in Cryo-Em on Pi Stack Hugo en-us Tue, 09 Jun 2026 00:00:00 +0000 https://www.pistack.xyz/posts/2026-06-09-self-hosted-cryo-em-image-processing-relion-eman2-guide/ Tue, 09 Jun 2026 00:00:00 +0000 https://www.pistack.xyz/posts/2026-06-09-self-hosted-cryo-em-image-processing-relion-eman2-guide/ <h2 id="introduction">Introduction</h2> <p>Cryo-electron microscopy (cryo-EM) has revolutionized structural biology, enabling atomic-resolution determination of protein structures without crystallization. The 2017 Nobel Prize in Chemistry recognized this breakthrough, and since then, cryo-EM facilities have proliferated worldwide. However, the computational challenge is immense — a single cryo-EM dataset can be 5-10 TB of micrograph movies, requiring days or weeks of GPU-accelerated processing.</p>