https://www.pistack.xyz/tags/calcium-imaging/ Recent content in Calcium-Imaging on Pi Stack Hugo en-us Sat, 13 Jun 2026 00:00:00 +0000 https://www.pistack.xyz/posts/2026-06-13-self-hosted-calcium-imaging-analysis-suite2p-caiman-minian/ Sat, 13 Jun 2026 00:00:00 +0000 https://www.pistack.xyz/posts/2026-06-13-self-hosted-calcium-imaging-analysis-suite2p-caiman-minian/ <h2 id="introduction">Introduction</h2> <p>Calcium imaging is a cornerstone technique in modern neuroscience, allowing researchers to monitor the activity of hundreds to thousands of neurons simultaneously in living tissue. Using genetically encoded calcium indicators (like GCaMP) or synthetic dyes, changes in fluorescence intensity reveal when neurons fire action potentials. But raw calcium imaging movies — typically gigabytes to terabytes of data — require sophisticated computational pipelines to extract meaningful neural activity.</p>